DETECTION OF SECONDARY-AMINES ON SOLID-PHASE

The detection of secondary amino groups in building blocks attached to solid phase using tetrachlorobenzoquinone (chloranil) was re-examined . A new procedure, about ten times move sensitive than the original me thod, has been developed. Except for N(p-tolyl)glycine, all secondary amino groups that were examined were detected reliably in a substituti on range down to 2.8 mu eq/g. Protected imidazole in His(Trt) did not interfere with the detection method.