THE SEQUENCE OF 24-CENTER-DOT-3 KB FROM CHROMOSOME-X REVEALS 5 COMPLETE OPEN READING FRAMES, ALL OF WHICH CORRESPOND TO NEW GENES, AND A TANDEM INSERTION OF A TY1 TRANSPOSON
M. Zagulski et al., THE SEQUENCE OF 24-CENTER-DOT-3 KB FROM CHROMOSOME-X REVEALS 5 COMPLETE OPEN READING FRAMES, ALL OF WHICH CORRESPOND TO NEW GENES, AND A TANDEM INSERTION OF A TY1 TRANSPOSON, Yeast, 11(12), 1995, pp. 1179-1186
We have determined the complete nucleotide sequence of a 24.3 kb segme
nt from chromosome X carried by the cosmid pEJ103. The sequence encode
s five complete open reading frames (ORFs), none of which correspond t
o previously described genes; however, four of these ORFs display inte
resting similarities with sequences present in the databanks. The sequ
ence also contains a tandem insertion of a Ty1 element. An investigati
on of the Ty1 polymorphism in other strains has revealed that the orig
inal insertion occurred within an ORF. Finally, the structure of the T
y1 repeat suggests a mechanism by which it may have been generated. Th
e sequence has been deposited in the EMBL data library under the Acces
sion Number X87297 for the complete sequence and X87298 for the Delta
ty1 version.