THE SEQUENCE OF AN 11-CENTER-DOT-1 KB FRAGMENT ON THE LEFT ARM OF SACCHAROMYCES-CEREVISIAE CHROMOSOME-VII REVEALS 6 OPEN READING FRAMES INCLUDING NSP49, KEM1 AND 4 PUTATIVE NEW GENES

We report the sequence of an 11.1 kb fragment located on the left arm of chromosome VII of Saccharomyces cerevisiae. By sequence analysis we have detected six open reading frames (ORFs) longer than 300 bp, whic h cover 87% of the entire sequence. ORF G1645 is 100% identical to the KEM1 gene, also identified as DST2, XRN1, SEP1 and RAR5, while G1648 is 100% identical to the NSP49 or NUP49 gene. ORF G1642 shares some id entity with a hypothetical protein of Caenorhabditis elegans, while th e other four ORFs show no significant homology to known proteins. The sequence has been submitted to the EMBL data library under Accession N umber X84705.