A 43-CENTER-DOT-5-KB SEGMENT OF YEAST CHROMOSOME-XIV, WHICH CONTAINS MFA2, MEP2, CAP SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 AND CPT1, PREDICTS AN ADENOSINE-DEAMINASE GENE AND 14 NEW OPEN READING FRAMES/

A 43,481 bp fragment from the left arm of chromosome XIV of Saccharomy ces cerevisiae was sequenced. A gene for tRNA(phc) and 23 non-overlapp ing open reading frames (ORFs) were identified, seven of which corresp ond to known yeast genes: MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1. One ORF may correspond to the yet unindentified yeast adenosine deaminase gene. Among the 15 other ORFs, four exhibit known signatures, which include a protein tyrosine phosphatase, a cytoskelet on-associated protein and two ATP-binding proteins, four have similari ties with putative proteins of yeast or proteins from other organisms and seven exibit no significant similarity with amino acid sequences d escribed in data banks. One ORF is identical to yeast expressed sequen ce tags (EST) and therefore corresponds to an expressed gene. Six ORFs present similarities to human dbESTs, thus identifying motifs conserv ed during evolution. Nine ORFs are putative transmembrane proteins. In addition, one overlapping and three antisense ORFs, which are not lik ely to be functional, were detected. The sequence has been deposited i n the EMBL data bank under Accession Number Z46843.