CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF ARYLESTERASE FROM VIBRIO-MIMICUS

Authors
MUSAYEV FN LEE YL SHAW JF LIAW YC
Citation
Fn. Musayev et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF ARYLESTERASE FROM VIBRIO-MIMICUS, Protein science, 4(9), 1995, pp. 1931-1933
Citations number
18
Categorie Soggetti
Biology
Journal title
Protein science → ACNP
ISSN journal
09618368
Volume
4
Issue
9
Year of publication
1995
Pages
1931 - 1933
Database
ISI
SICI code
0961-8368(1995)4:9<1931:CAPCAO>2.0.ZU;2-7
Abstract
Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperatu re for 2 months. The present crystals diffract up to 2.2 Angstrom reso lution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) Angstrom, b = 53.46(1) Angstrom, c = 65.79(1) Angstr om, and beta = 106.54(1)degrees. There are two molecules of molecular weight 22 kDa in an asymmetric unit with a solvent content of 43%.