APPLICATION OF MONTE-CARLO INTEGRATION TO 3-DIMENSIONAL FLUORESCENCE SPECTROMETRY

In this paper, the sensitivity of three-dimensional fluorescence spect rometry (TDFS) is studied by using the total fluorescence intensity, c alculated by Monte Carlo integration, instead of conventional single-p oint intensity. It has been proven that the sensitivity of the total f luorescence intensity method is nearly one hundred times better than t hat of the single-point intensity method for the determinations of flu orescein and tryptophan. The mechanism of the method has also been stu died. Index Headings: Fluorescence; Three-dimensional fluorescence spe ctrometry; Chemometrics; Monte Carlo techniques; Monte Carlo integrati on; Fluorescein; Tryptophan.