PHOTOPRODUCTION OF THE AZIDYL RADICAL FROM THE AZIDE ANION ON THE OXIDIZING SIDE OF PHOTOSYSTEM-II AND SUPPRESSION OF PHOTOOXIDATION OF TYROSINE-Z BY THE AZIDYL RADICAL

Citation
K. Kawamoto et al., PHOTOPRODUCTION OF THE AZIDYL RADICAL FROM THE AZIDE ANION ON THE OXIDIZING SIDE OF PHOTOSYSTEM-II AND SUPPRESSION OF PHOTOOXIDATION OF TYROSINE-Z BY THE AZIDYL RADICAL, Plant and Cell Physiology, 36(6), 1995, pp. 1121-1129
Citations number
41
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00320781
Volume
36
Issue
6
Year of publication
1995
Pages
1121 - 1129
Database
ISI
SICI code
0032-0781(1995)36:6<1121:POTARF>2.0.ZU;2-V
Abstract
Azide ions inhibited O-2 evolution in PSII membranes from spinach in a time-dependent manner in the light until all activity disappeared. Il lumination in the presence of azide (azide-phototreatment) irreversibl y inhibited the following processes: (1) both the oxidation of water a nd the electron transport between the redox-active tyrosine 161 of the D1 protein (Y-Z) and the secondary quinone electron acceptor (Q(B)) s ite, to the same extent; (2) the donation of electrons to the primary quinone electron acceptor (Q(A)), as measured by monitoring the maximu m variable fluorescence of Chl; and (3) the photoproduction of the Y-Z radical (Y-Z(.)). Thus, the primary site of inhibition appeared to li e between Y-Z and Q(A). On illumination of Tris-treated PSII membranes in the presence of azide, production of the azidyl radical was observ ed by spin-trapping ESR. Yield of Y-Z(.) in Tris-treated membranes on illumination was suppressed by azide. Electron transport from Y-Z to Q (B) in Tris-treated membranes was inhibited only when the azidyl radic al was photoproduced, and it was inhibited more rapidly than it was in the oxygenic PSII membranes. These results indicate that the azidyl r adical was produced via a univalent oxidation of azide by Y-Z(.) and t hat it irreversibly inhibited the electron transport from Y-Z to Q(A) in Tris-treated membranes. Although the azidyl radical was undetectabl e in the oxygenic PSII membranes, probably due to steric interference by the peripheral proteins of water-oxidizing complex with the access of the spin-trapping reagent to the production site of the radical, th e participation of the azidyl radical in the inhibition of the oxygeni c PSII membranes is suggested since simultaneous occurrence of both el ectron transport and azide was required for the inhibition. Possible i nhibitory mechanisms and the target sites of azidyl radical are discus sed.