PHOTOPRODUCTION OF THE AZIDYL RADICAL FROM THE AZIDE ANION ON THE OXIDIZING SIDE OF PHOTOSYSTEM-II AND SUPPRESSION OF PHOTOOXIDATION OF TYROSINE-Z BY THE AZIDYL RADICAL
K. Kawamoto et al., PHOTOPRODUCTION OF THE AZIDYL RADICAL FROM THE AZIDE ANION ON THE OXIDIZING SIDE OF PHOTOSYSTEM-II AND SUPPRESSION OF PHOTOOXIDATION OF TYROSINE-Z BY THE AZIDYL RADICAL, Plant and Cell Physiology, 36(6), 1995, pp. 1121-1129
Azide ions inhibited O-2 evolution in PSII membranes from spinach in a
time-dependent manner in the light until all activity disappeared. Il
lumination in the presence of azide (azide-phototreatment) irreversibl
y inhibited the following processes: (1) both the oxidation of water a
nd the electron transport between the redox-active tyrosine 161 of the
D1 protein (Y-Z) and the secondary quinone electron acceptor (Q(B)) s
ite, to the same extent; (2) the donation of electrons to the primary
quinone electron acceptor (Q(A)), as measured by monitoring the maximu
m variable fluorescence of Chl; and (3) the photoproduction of the Y-Z
radical (Y-Z(.)). Thus, the primary site of inhibition appeared to li
e between Y-Z and Q(A). On illumination of Tris-treated PSII membranes
in the presence of azide, production of the azidyl radical was observ
ed by spin-trapping ESR. Yield of Y-Z(.) in Tris-treated membranes on
illumination was suppressed by azide. Electron transport from Y-Z to Q
(B) in Tris-treated membranes was inhibited only when the azidyl radic
al was photoproduced, and it was inhibited more rapidly than it was in
the oxygenic PSII membranes. These results indicate that the azidyl r
adical was produced via a univalent oxidation of azide by Y-Z(.) and t
hat it irreversibly inhibited the electron transport from Y-Z to Q(A)
in Tris-treated membranes. Although the azidyl radical was undetectabl
e in the oxygenic PSII membranes, probably due to steric interference
by the peripheral proteins of water-oxidizing complex with the access
of the spin-trapping reagent to the production site of the radical, th
e participation of the azidyl radical in the inhibition of the oxygeni
c PSII membranes is suggested since simultaneous occurrence of both el
ectron transport and azide was required for the inhibition. Possible i
nhibitory mechanisms and the target sites of azidyl radical are discus
sed.