Ct. Nugent et al., DIFFERENCES IN THE RECOGNITION OF CTL EPITOPES DURING PRIMARY AND SECONDARY RESPONSES TO HERPES-SIMPLEX VIRUS-INFECTION IN-VIVO, Cellular immunology, 165(1), 1995, pp. 55-64
The immune response to HSV infection in C57BL/6 mice includes a CTL po
pulation that recognizes the virion envelope glycoprotein gB. However,
previous studies showed that CTL specific for other viral determinant
s were also responding to HSV infection. These studies demonstrate tha
t an additional determinant is the HSV immediate-early protein ICP27.
During the primary response, both gB- and ICP27-specific CTL were dete
cted in the draining lymph node. In response to reinfection, ICP27-spe
cific CTL were present early in the lymph node, while the appearance o
f gB-specific CTL activity was delayed. Analysis of the primary amino
acid sequence of ICP27 predicted two potential K-b-binding epitopes, o
ne of which sensitized uninfected cells for lysis by HSV-specific CTL.
In addition, ICP27 epitope-specific CTL activity was detected in the
splenic memory CTL pool. These results show that CTL which recognize d
ifferent antigens may also exhibit differences in how they respond to
HSV reinfection in vivo. (C) 1995 Academic Press, Inc.