A REPORT ON THE DEMONSTRATION OF MICROFILARIAE OF BRUGIA-MALAYI IN THE BRAIN OF AN EXPERIMENTAL ANIMAL HOST, MASTOMYS-NATALENSIS

A report on the demonstration of microfilariae of Brugia malayi in the brain of an experimental animal host, Mastomys natalensis. internatio nal Journal for Parasitology 25: 1123-1126. The brain tissues of micro filaraemic animals, Mastomys natalensis, which were earlier inoculated (s.c.) with Brugia malayi infective larvae (100 each) were examined f or the occurrence of Mf. This was done by staining squash preparations of the brain tissues which were cleared off from the vascular piamate r. Animals with blood Mf count of 50>/per 20 cu. mm were found to harb our Mf in the brain tissues. The Mf count in the brain varied from 5-8 6/81 cu. mm (sum of Mf detected in 3 tissue pieces, each of 27 cu. mm and medulla oblongata). The presence of Mf in the brain was confirmed by its detection in 20-mu m-thick cryosections of the tissue. Also, fi ne needle aspirates of cerebral hemispheres of an animal showed live M f.