LARGE-CELL ANAPLASTIC NON-HODGKINS-LYMPHOMA ORIGINATING IN DONOR CELLS AFTER ALLOGENIC BONE-MARROW TRANSPLANTATION

Second neoplasms after allogenic bone marrow transplantation (BMT) occ ur in donor cells; however, host origin generally cannot be excluded. Using fluorescence in situ hybridization (FISH) the origin of the mali gnant population can be proven indisputably. In a female patient with CD30(+) large-cell anaplastic non-Hodgkin's lymphoma (LCAL) after BMT with an HLA-identical brother donor, FISH using anti-CD30 immunocytoch emistry in combination with anti-Y- and anti-EBV RNA probes was applie d. In pathological lymph nodes the majority of cells were of donor typ e (Y). CD30-positive cells were Y-positive; these cells were also EBV- positive. Using FISH and immunocytochemistry we have demonstrated conv incingly that this, possibly EBV-induced, LCAC originated in donor cel ls.