CLONING AND CHROMOSOMAL MAPPING OF 3 NOVEL GENES, GPR9, GPR10, AND GPR14 ENCODING RECEPTORS RELATED TO INTERLEUKIN-8, NEUROPEPTIDE-Y, AND SOMATOSTATIN RECEPTORS

We employed the polymerase chain reaction and genomic DNA library scre ening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR1 4. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR 10 and GPR14 are intronless within their coding regions, while GPR9 co ntains at least one intron. The receptor encoded by GPR9 shares the hi ghest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% ove rall and 51% in the transmembrane domains). GPR10 encodes a receptor t hat shares highest identity with the neuropeptide Y receptor (31% over all and 46% in the transmembrane domains). The receptor encoded by GPR 14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hy bridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26. (C) 1995 Academic Press, Inc.