YAC AND COSMID CONTIGS SPANNING THE BATTEN-DISEASE (CLN3) REGION AT 16P12.1-P11.2

A yeast artificial chromosome (YAC) contig has been constructed in 16p 12.1-p11.2 that encompasses three loci (D16S288, D16S299, and D16S298) closely linked to the gene causing fatten disease or juvenile-onset n euronal ceroid lipofuscinosis (CLN3). The physical map has been ordere d using 42 sequence tagged sites. Four genes, interleukin-4 receptor ( IL4R), phenol-preferring phenol sulfotransferase (STP), monoamine-pref erring phenol sulfotransferase (STM), and sialophorin (SPN), have been mapped to the YAC contig. A partial genomic restriction map has been constructed to confirm the order and distances between D16S288 and STM . This part of the YAC contig is represented in eight cosmid contigs. One of these contains D16S298, predicted to be the locus closest to CL N3. The overlapping genomic clones are a valuable resource for cloning the Batten gene (CLN3) and other genes in the region. (C) 1995 Academ ic Press, Inc.