REGULATION OF DYNEIN-DRIVEN MICROTUBULE SLIDING BY AN AXONEMAL KINASEAND PHOSPHATASE IN CHLAMYDOMONAS FLAGELLA

The following is a summary of physiological and pharmacological studie s of the regulation of dynein-driven microtubule sliding in Chlamydomo nas flagella. The experimental basis for the study is described, and d ata indicating that an axonemal cAMP-dependent protein kinase can regu late inner arm dynein activity are reviewed. In addition, preliminary data are summarized indicating that an axonemal type 1 phosphatase can also regulate dynein-drive microtubule sliding velocity. It is predic ted that the protein kinase, phosphatase, and an inner dynein arm comp onent form a regulatory complex in the axoneme. (C) 1995 Wiley-Liss, I nc.