N. Bargmannleyder et al., A COMPARISON OF LC AND SFC FOR CELLULOSE-DERIVED AND AMYLOSE-DERIVED CHIRAL STATIONARY PHASES, Chirality, 7(5), 1995, pp. 311-325
This paper presents a systematic comparison of liquid chromatography (
LC) and supercritical fluid chromatography (SFC) for Chiralcel OD and
Chiralpak AD chiral stationary phases (CSPs), performed using various
chiral compounds having a known or potential pharmaceutical activity.
The chiral recognition mechanisms involved in LC and SFC for the enant
iomeric separation of beta-blockers have been studied more particularl
y. As a general rule, it appears that the presence of polar functions,
like primary or secondary hydroxyl or amine functions, may result in
marked discrepancies in selectivity between LC and SFC, This result is
peculiar to cellulose- and amylose-derived CSPs, for which the intera
ctions involved in chiral recognition mechanism are not always well ba
lanced, contrary to what happens for independent CSPs. In the case of
chiral resolution of polar solutes or polymer-type CSPs, the analyst s
hould try both the LC and SFC techniques to be able to choose the more
stereoselective one. (C) 1995 Wiley-Liss, Inc.