Gek. Novotny et al., ULTRASTRUCTURAL QUANTITATIVE-ANALYSIS OF THE INNERVATION OF AXILLARY LYMPH-NODES IN JUVENILE AND OLD RATS, The Anatomical record, 243(2), 1995, pp. 223-233
Background: Earlier studies have shown that the innervation of axillar
y lymph nodes is increased in old animals, compared to juvenile rats,
The question thus arose, whether changes are also observable at an ult
rastructural level for varicosities which form the actual transmission
units, Methods: Ensheathed (axons) and unensheathed axonal profiles (
open areas), were quantified with their spatial relationships to cells
at the ultrastructural level in the axillary lymph nodes of juvenile
(< 6 weeks) and old (> 2 years) Wistar rats. Results: In both groups o
f animals the majority of open areas, irrespective of their content of
vesicles, lie at distances of more than 1,000 nm from cells in the pl
ane of the section, Almost all open areas, located within 1,000 nm of
a cell, are related to reticular cells, a small minority to plasma cel
ls, and even fewer to lymphocytes. In the old animals there is a highl
y significant increase in the total number of sections of open areas w
ith and without vesicles per unit area of medulla, The number of open
areas related to cells does not change significantly in the old animal
s, as also the percentage of profiles containing vesicles. Only the pe
rcentages of open areas with and without vesicles related to plasma ce
lls increase significantly in the old animals, This change is parallel
ed by a highly significant increase in the percentage volume of plasma
cells in the old animals, On average, the profiles related to cells a
re closer to the cells in the older animals, Conclusions: The observed
changes indicate that the innervation of lymph nodes of the rat is ab
solutely increased in the old animals, but that the relations between
the various categories of profiles and their relations to cells tend t
o remain constant. (C) 1995 Wiley-Liss, Inc.