SUCCESSION OF CELLULAR-STATES IN A SALMONELLA-TYPHIMURIUM POPULATION DURING STARVATION IN ARTIFICIAL SEAWATER MICROCOSMS

Authors
JOUX F LEBARON P TROUSSELLIER M
Citation
F. Joux et al., SUCCESSION OF CELLULAR-STATES IN A SALMONELLA-TYPHIMURIUM POPULATION DURING STARVATION IN ARTIFICIAL SEAWATER MICROCOSMS, FEMS microbiology, ecology, 22(1), 1997, pp. 65-76
Citations number
32
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology, ecology → ACNP
ISSN journal
01686496
Volume
22
Issue
1
Year of publication
1997
Pages
65 - 76
Database
ISI
SICI code
0168-6496(1997)22:1<65:SOCIAS>2.0.ZU;2-L
Abstract
The succession of the physiological states of Salmonella typhimurium c ells under starvation-survival conditions in artificial seawater was i nvestigated by now cytometry and epifluorescence microscopy using dire ct examination of different cell functions. Measurements of substrate responsiveness (DVC method), real and potential respiration (redox dye 5-cyano-2,3-ditolyl tetrazolium chloride, CTC), membrane permeability (BacLight(TM) kit), as well as DNA content (Hoechst 33342 staining) r eveal an important heterogeneity within the population, and suggest a progressive physiological cell alteration throughout the starvation pr ocess. The transition steps between culturable and prelytic cells are described.