GENERATION OF GIANT PROTOPLASTS OF ESCHERICHIA-COLI AND AN INNER-MEMBRANE ANION-SELECTIVE CONDUCTANCE

Authors
KUBALSKI A
Citation
A. Kubalski, GENERATION OF GIANT PROTOPLASTS OF ESCHERICHIA-COLI AND AN INNER-MEMBRANE ANION-SELECTIVE CONDUCTANCE, Biochimica et biophysica acta. Biomembranes, 1238(2), 1995, pp. 177-182
Citations number
34
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Biomembranes → ACNP
ISSN journal
00052736
Volume
1238
Issue
2
Year of publication
1995
Pages
177 - 182
Database
ISI
SICI code
0005-2736(1995)1238:2<177:GOGPOE>2.0.ZU;2-5
Abstract
Established methods were modified and combined to generate unilamellar giant protoplasts in order to study the electric events on the cytopl asmic membrane of Escherichia coli with patch-clamp technique. The act ivities of many types of conductances were encountered, one of them is characterized here. This channel conductance is 109 pS in 150 mM KCl; it prefers anions, it is highly voltage dependent and is blocked by m icromolar concentrations of anthracene-9-carboxylic acid.