Results of previously published experiments based on open-top chambers
and field exposure systems with seedlings, mature grafts and physiolo
gically mature trees (<13m tall) of Sitka spruce exposed to pH 2.5 mis
t are discussed briefly for the purpose of identifying reproducible bi
oindicators of acid mist induced perturbation, i.e. physiological dist
urbance causing stress (reduction in performance). None of the bioindi
cators studied, i.e. visible injury, foliar nutrient concentrations, i
on leakage with and without freezing, and growth responded consistentl
y to treatment. Substantial treatment by environment interactions were
detected in treatment effects on visible injury and foliar nutrient c
oncentrations. But the enhancement of ion leakage from unfrozen shoots
and changes in foliar nutrient concentrations in treated compared wit
h untreated shoots reliably indicated acid mist-induced stress.