Studies by light and scanning-electron microscopy in combination with
specific isolation techniques revealed the presence of soil-borne Phyt
ophthora - species in declining oak, beech and other forest trees. The
sequence of histopathological reactions in infected roots and distinc
t fungal structures found in necrotic tissues are documented. Specific
laboratory procedures used for the detection and identification of Ph
ytophthora are presented. Pathogenicity of the isolated Phytophthora -
species is shown by common tests of soil infestation and stem inocula
tion. The possible role of predisposing and contributing factors leadi
ng to this type of decline is discussed with emphasis on climate chang
e and excess nitrogen.