MOLECULAR AND CYTOGENETIC MAPPING OF PLANT GENOMES

Three principal approaches are used in our laboratory to analyze plant genomes: (i) The construction of high density molecular maps using a refined microdissection procedure to construct chromosomal and subchro mosomal libraries. (ii) Synteny analysis: The Aegilops-based deletion mapping system in wheat that has led to the construction of a high-den sity physical consensus map of wheat. The integration of wheat, barley and oat RFLP markers has proven the colinearity between the wheat A-, B- and D-genomes, the H-genome of barley, and the E-genome of Agropyr on. (iii) Gene mapping in situ, and chromosomes at high resolution: Se nsitivity enhancement of fluorescence in situ hybridization (FISH) and an efficient preparation of plant chromosomes has been developed. A t andemly amplified repetitive sequence element from microdissected barl ey chromosomes has enabled the karyotyping of individual Gramineae gen omes in a single step. These sequences are also useful for double or t riple hybridization-experiments. The low copy genes for the: storage p roteins Sec-1 and Hor-B have been topographically mapped on the satell ite of chromosome 1R of rye and the syntenic locus on barley chromosom e 1H.