IDENTIFICATION OF POTENTIAL CYTOGENETICAL AND BIOCHEMICAL MARKERS IN BIOINDICATION OF COMMON OAK FORESTS

Cytogenetical and biochemical investigations of common oak (Quercus ro bur L.) were applied in order to find suitable markers in bioindicatio n of oak populations. Quercus robur karyotype from investigated popula tions consisted of 24 chromosomes including a satellite pair. One or t wo small spherical chromatin fragments, ''B-chromosomes'' were present in some prophase or metaphase cells. Giemsa C-banding, chromomycine A (3)/DAPI reverse staining and silver impregnation showed the same sign al on spherical fragments as on the NORs of satellite chromosomes indi cating that ''B-chromosomes'' were broken NORs. They were entirely het erochromatic, G-C rich, showing AgNO3 positive reaction and they could be used as markers in bioindication of oak populations. Protein bands of about 60 and 95 kDa were distinct in the leaves of heavily damaged trees. Due to its stability isoperoxidases pattern of dormant buds an d young leaves might be convenient tissue for biochemical bioindicatio n.