DETECTION OF INTERSPECIES HYBRIDS IN PLANTS BY FLUORESCENCE IN-SITU HYBRIDIZATION, USING TOTAL GENOMIC DNA AS A PROBE

Total genomic DNA probe preparation and the non-isotopic biotin probe labelling technique are described. The use of an excess of unlabelled competitor DNA from a related plant species gives more constant result s in determing whole plant genomes with in situ experiments on plant h ybrids. Results of fluorescence in situ hybridization for detecting al ien rye chromatin and localisation on metaphase chromosomes and interp hase nuclei in the bread wheat cultivar 'Yugoslavia'' which carries a 1BL.1RS translocation are shown.