USE OF IMMOBILIZED BACTERIA TO TREAT INDUSTRIAL WASTE-WATER CONTAINING A CHLORINATED PYRIDINOL

Pseudomonas sp. strain M285 immobilized on diatomaceous earth beads wa s used to remove 3,5,6-trichloro-2-pyridinol (TCP) from industrial was tewater. Batch studies showed that immobilized Pseudomonas sp. strain M285 mineralized [2,6-C-14]TCP rapidly; about 75% of the initial radio activity was recovered as (CO2)-C-14. Transformation of TCP was inhibi ted by high concentrations of salt, and addition of osmoprotectants (p roline and betaine at 1 mM) did not reduce the adverse effect of salt. TCP-containing wastewater (60-140 mg/l) was passed through columns co ntaining immobilized Pseudomonas sp. strain M285 at increasing flow ra tes and increasing TCP concentrations; TCP removal of 80%-100% was ach ieved. Addition of nutrients, such as glucose and yeast extract, retar ded TCP degradation. Growing cell cultures were found to be better ino cula for immobilization than resting cells.