INDUCTION FROM METALLOTHIONEINE THROUGH C ISPLATIN

An increase in the expression of metallothioneine (MT) as a possible r esistance mechanism of secondary cisplatin resitance of malign tumors is under investigation. MT is an ubiquitous, low-molecular metal prote in that can bind up to 7 g/mol protein. MT is incited by heavy metals and also cytostatika, e.g. cisplatin. A resistant secondary cell line CP3, selected from a human urothel carcinoma cell RT 112, was first pr ogressively exposed to increasing concentrations of cisplatin. A monoc lonal cell line K1 was also isolated. The MT II content of the cells w as evaluated with the aid of capillary zone electrophoresis. The conte nt of MT II in the cisplatin resistant cells had increased in comparis on with the parent cell line RT 112.