ENZYMATIC-HYDROLYSIS OF CRAYFISH PROCESSING BY-PRODUCTS

Ten commercial proteases (neutral and alkaline) were evaluated for hyd rolysis of crayfish processing by-products (CPBs). Hydrolysis conditio ns were optimized for the alkaline protease Optimase(TM) APL-440 by re sponse surface methodology (RSM). Two model equations were proposed wi th regard to effects of pH, temperature (T), time (t), enzyme/substrat e (E/S) ratio, and substrate concentration (S) on the amount of 0.3M T CA soluble peptides (TSP) and degree of hydrolysis (DH). Interaction e ffects between pH and T were observed (p<0.001). Based on TSP, optimum hydrolysis conditions were determined to be pH 8-9, 65 degrees C, 2.5 fir reaction time, 75%(w/v) substrate concentration, and 0.3% (v/w) e nzyme.