The hydrocarbons of honeybee comb wax vary significantly between colon
ies. This variation is explained in part by genetic (familial) differe
nces among colonies. Even though significant differences in wax hydroc
arbons exist among families, there is a high level of consistency with
in and among families in a correlation analysis, indicating structural
constancy in comb wax. The significance of these results in interpret
ing the potential role of comb wax in the nestmate recognition system
of the honeybee is discussed.