An investigation of the phylogenetic relationships of a number of cart
ilaginous fish based on the use of the immunological properties of the
iron-binding serum protein, transferrin, is presented. Data on immuno
logical distance and the rate of amino acid substitution are used to p
roduce a dendrogram. The tree confirms the close relationships of the
holocephalan species examined which are a sister group of other neosel
achians. The tree also indicates that the squaloids and galeoids are s
eparate groups and that Heterodontus belongs with the galeoids. It is
also clear that the divergence between the squaloids, galeoids (includ
ing Heterondontus) and the Holocephali is an ancient one and that the
rajids probably diverged from this basal stock rather than any more re
cent squaloid/squatinoid/galeoid grouping.