A NEW SENSITIVE AND SIMPLE METHOD FOR DETECTION OF CATECHOLAMINES FROM ADRENAL CHROMAFFIN CELLS

Citation
Al. Ghindilis et al., A NEW SENSITIVE AND SIMPLE METHOD FOR DETECTION OF CATECHOLAMINES FROM ADRENAL CHROMAFFIN CELLS, Die Pharmazie, 50(9), 1995, pp. 599-600
Citations number
6
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
ISSN journal
00317144
Volume
50
Issue
9
Year of publication
1995
Pages
599 - 600
Database
ISI
SICI code
0031-7144(1995)50:9<599:ANSASM>2.0.ZU;2-6
Abstract
A biosensor was used for the analysis of catecholamines in media and l ysates of cultured bovine adrenal chromaffin cells, The sensor is comp osed of coimmobilised laccase and glucose dehydrogenase coupled with a n oxygen electrode, using the catalytic effect of cate cholamines for glucose oxidation in this system, The analysis time is almost 5 min. T he correlation between the biosensor and HPLC determination is 0.99.