FTS INSERTIONAL MUTANT OF SALMONELLA-TYPHIMURIUM

A temperature-sensitive filamentation (fts) SalmonellA typhimurium mut ant was isolated after transposon mutagenesis with mini-Tn10dTc. The m utant was unable to form colonies after 20 h incubation at 37 degrees C on LB agar. Colonies appeared, however, after longer incubation at t he restrictive temperature. Filamentation affected only part of the ba cterial population. Rapid mapping using MudP22 hybrid phages revealed that the mutation, ftsD220, lies within minutes 68.5 and 73.6 on the g enetic map. Further analysis revealed that the ftsD220 mapped at min 7 3 and that it is linked to cysG (6%) and to aroB (39%). Complementatio n tests suggested that the ftsD220 mutation is not homologous to a Esc herichia coli ftsH mutation.