Random amplified polymorphic DNA (RAPD) markers were used to detect ch
imerism of citrus cultivars. Polymerase chain reaction conditions suit
able for discriminating citrus chimeras were determined. Primers that
produced consistent and repeatable bands that differed between the par
ental cultivars were chosen to create discriminating band patterns, Ou
r results show that selected 12-mer primers can be useful for identify
ing the four citrus chimeras tested using RAPD technology.