INACTIVATION OF THE DCC TUMOR-SUPPRESSOR GENE IN A B-CELL LYMPHOMA CELL-LINE WITH THE ALTERATION OF CHROMOSOME-18

A B-cell lymphoma cell line, designated KML-1, was established from pl eural effusion of a patient with non-Hodgkin's lymphoma of large cell type, The lymphoma arose in the pelvis and ran an aggressive clinical course. Chromosome analysis of the cell line exhibited a complex karyo type including the loss of chromosome 18. To evaluate the molecular ev ents in the cell line that may be associated with the development of t he lymphoma, we investigated the expression and/or alterations of seve ral classes of human genes, including oncogenes, tumor suppressor gene s, and cytokine genes. The expression of the DCC (deleted in colorecta l cancer) gene, located on the chromosome 18q21, was extremely reduced in KML-1 cell line, as compared with that in a normal spleen tissue a nd other 4 lymphoma cell lines by the reverse transcription-polymerase -chain-reaction (RT-PCR) method. This finding suggests that inactivati on of the DCC gene might play a role in the pathogenesis of the case o f lymphoma. (C) 1995 Wiley-Liss, Inc.