Jj. Rybczynski et al., CHANGES IN MORPHOGENIC POTENTIAL OF SHOOT REGENERATION FROM ROOT EXPLANT OF LOTUS-CORNICULATUS L SEEDLING, Acta Physiologiae Plantarum, 17(3), 1995, pp. 211-218
Experiments were carried out using seedling root explant of Lotus corn
iculatus L. (Birdsfoot trefoil) to describe morphogenic potential for
shoot regeneration of three explants originated from different parts o
f root. Seedlings were maintained on MS hormone-free medium. Explants
were isolated from 4, 8 and 16-day old seedlings. Explants cultured on
MS hormone-free medium regenerated shoots and roots. Buds were only f
ormed on proximal end of explants while roots on the distal end. Expla
nt position in root affects its morphogenic reponse. Explants located
proximally regenerated more of shoots than those originated from the d
istal end. The age of seedling did not modify the explant response on
in vitro conditions. In the presence of 0.1 mg/l 6-benzylaminopurine (
BAP) the number of regenerated plants was higher, because numerous mer
istematic zones formed in secondary cortex. In contrast to the explant
response on hormone-free medium, disturbances of explant polarity wer
e observed. Roots were not regenerated from explant tissue. Low BAP co
ncentration i.e. 0.1 mg/l in medium cancelled the effect of explant po
sition.