NOVEL COLORECTAL ADENOCARCINOMA-ASSOCIATED 40-KDA AND 47-KDA PROTEIN ANTIGENS RECOGNIZED BY ANTI-60-KDA HEAT-SHOCK PROTEIN ANTIBODY

Expression of 40- and 47-kDa proteins recognized by anti-60-kDa heat s hock protein antibody was investigated in colorectal adenocarcinoma ti ssue. In all cases of colorectal adenocarcinoma tissue (n = 9), 40- an d 47-kDa proteins were detected by immunoblot analysis. However, expre ssion of these two proteins was extremely low or was not expressed in control tissue. There was no correlation between the quantity of these two bands and histologic type or the location of the tumors. Our resu lts first demonstrate the accumulation of structurally altered 60-kDa heat shock protein-associated antigens in carcinoma tissue and suggest that these two proteins could be a possible candidate for tumor-speci fic 60-kDa heat shock protein-related protein antigens.