G. Morovjan et al., DETERMINATION OF GLYPHOSATE AND SOME RELATED-COMPOUNDS BY ION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of liquid chromatography, 18(16), 1995, pp. 3219-3232
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Anion exchange liquid chromatographic methods are described for the de
termination of N-phosphonomethyl-glycine (PMG), which is known also as
glyphosate, its monomethylester (PMG-Met), N,N-bis(phosphonomethyl)-g
lycine (bis-PMG), N-methyl, N-phosphonomethyl-glycine (PMG-NMet) and g
lycine (Gly). Five different anion exchange columns were tested for th
e separation of the above mentioned compounds. Effects of the eluent p
H, ionic strength and organic solvent composition were studied. It was
found that only some of the tested columns were applicable for the se
paration of the PMG and PMG-NMet critical pair, which may be due to th
e different structure, the ratio of the hydrophobic and hydrophilic su
rface area of the ion exchanger. Analytical performance of the methods
was investigated. Some examples are also shown for the application of
these described methods.