DETERMINATION OF GLYPHOSATE AND SOME RELATED-COMPOUNDS BY ION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Anion exchange liquid chromatographic methods are described for the de termination of N-phosphonomethyl-glycine (PMG), which is known also as glyphosate, its monomethylester (PMG-Met), N,N-bis(phosphonomethyl)-g lycine (bis-PMG), N-methyl, N-phosphonomethyl-glycine (PMG-NMet) and g lycine (Gly). Five different anion exchange columns were tested for th e separation of the above mentioned compounds. Effects of the eluent p H, ionic strength and organic solvent composition were studied. It was found that only some of the tested columns were applicable for the se paration of the PMG and PMG-NMet critical pair, which may be due to th e different structure, the ratio of the hydrophobic and hydrophilic su rface area of the ion exchanger. Analytical performance of the methods was investigated. Some examples are also shown for the application of these described methods.