PRODUCTION OF TRICHODERMA-REESEI CELLULASES ON GLUCOSE-CONTAINING MEDIA

The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose containing me dium, The expression of the proteins was driven by the promoters of th e elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1 . The cDNA1 promoter gave the best yields. The highest amounts of cell obiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter , accounted for more than half of the total protein secreted by the fu ngus, The levels obtained with the tef1 promoter were 20 to 50 times l ower,