S. Kilvington et J. Beeching, DEVELOPMENT OF A PCR FOR IDENTIFICATION OF NAEGLERIA-FOWLERI FROM THEENVIRONMENT, Applied and environmental microbiology, 61(10), 1995, pp. 3764-3767
A species-specific PCR for the identification of Naegleria fowleri was
developed. In sensitivity studies, 10 trophozoites or cysts and 1 tro
phozoite or cyst could be detected after 35 and 45 cycles, respectivel
y. In conjunction with a rapid DNA isolation method, this PCR was used
to identify N. fowleri directly from primary cultures of environmenta
l samples.