DEVELOPMENT OF A PCR FOR IDENTIFICATION OF NAEGLERIA-FOWLERI FROM THEENVIRONMENT

Authors
KILVINGTON S BEECHING J
Citation
S. Kilvington et J. Beeching, DEVELOPMENT OF A PCR FOR IDENTIFICATION OF NAEGLERIA-FOWLERI FROM THEENVIRONMENT, Applied and environmental microbiology, 61(10), 1995, pp. 3764-3767
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
10
Year of publication
1995
Pages
3764 - 3767
Database
ISI
SICI code
0099-2240(1995)61:10<3764:DOAPFI>2.0.ZU;2-S
Abstract
A species-specific PCR for the identification of Naegleria fowleri was developed. In sensitivity studies, 10 trophozoites or cysts and 1 tro phozoite or cyst could be detected after 35 and 45 cycles, respectivel y. In conjunction with a rapid DNA isolation method, this PCR was used to identify N. fowleri directly from primary cultures of environmenta l samples.