Manganese (Mn) is an essential element, the deficiency or excess of wh
ich is known to cause neurotoxicity in experimental animals and man. T
he mechanism of action of Mn neurotoxicity is still unclear. The prese
nt study was designed to evaluate whether in vitro or in vivo exposure
to Mn produced reactive oxygen species (ROS). We also sought to deter
mine if a single injection of Mn produces changes in monoamines concen
tration in different regions of rat brain. Adult Sprague-Dawley rats w
ere dosed with 0, 50 or 100 mg/kg, ip with either MnCl2 (Mn+2) or MnOA
c (Mn+3) and were sacrificed 1 h after the dose was administered. Brai
ns were quickly removed and dissected for neurochemical analysis. ROS
were measured by a molecular probe, 2',7'-dichlorofluorescein diacetat
e (DCFH-DA), and monoamines and their metabolites were measured by HPL
C/EC. In vitro exposure to MnCl2 (1-1000 mu M) produced dose-dependent
increases of ROS in striatum whereas MnOAc produced similar increases
at much lower concentrations (1-100 mu M) In vivo exposure to MnOAc (
Mn+3) produced significant increases of ROS in caudate nucleus and hip
pocampus, whereas MnCl2 (Mn+2) produced significant effects only in hi
ppocampus. Concentrations of dopamine, serotonin and their metabolites
(DOPAC, HVA and 5-HIAA) were not altered with acute injections of eit
her MnCl2 or MnOAc. These data suggest that both divalent and trivalen
t manganese induce ROS, however, Mn+3 is an order of magnitude more po
tent than Mn+2. (C) 1995 Academic Press Limited