SYNTHESIS OF 2-DEOXY-GLUCOOLIGOSACCHARIDES THROUGH CONDENSATION OF 2-DEOXY-D-GLUCOSE BY GLUCOAMYLASE AND ALPHA-GLUCOSIDASE

Glucoamylases from Aspergillus niger and Rhizopus niveus catalyzed con densation of 2-deoxy-D-glucose (dGlc) to yield deoxy-glucooligosacchar ides with polymerization degrees of 2-5, The enzymes also gave a small amount of products from 3-deoxy-D-glucose, but no products from 6-deo xy-D-glucose. A. niger alpha-glucosidase also catalyzed condensation o f dGlc, while Torula and Saccharomyces alpha-glucosidases had low acti vity, alpha-1,4-, 1,6-, and 1,3-linked deoxy-glucobioses were isolated and identified as the products of A. niger glucoamylase and A, niger alpha-glucosidase, In the reaction of the glucoamylase, 1,4- and 1,3-l inked saccharides decreased with an increase of 1,6-linked one, A, nig er alpha-glucosidase produced alpha-1,6-linked disaccharide predominan tly during the whole course of the reaction.