CLONING AND COMPLETE SEQUENCE OF THE DNA POLYMERASE-ENCODING GENE (BSTPOLI) AND CHARACTERIZATION OF THE KLENOW-LIKE FRAGMENT FROM BACILLUS-STEAROTHERMOPHILUS

Authors
PHANG SM TEO CY LO E WONG VWT
Citation
Sm. Phang et al., CLONING AND COMPLETE SEQUENCE OF THE DNA POLYMERASE-ENCODING GENE (BSTPOLI) AND CHARACTERIZATION OF THE KLENOW-LIKE FRAGMENT FROM BACILLUS-STEAROTHERMOPHILUS, Gene, 163(1), 1995, pp. 65-68
Citations number
21
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
163
Issue
1
Year of publication
1995
Pages
65 - 68
Database
ISI
SICI code
0378-1119(1995)163:1<65:CACSOT>2.0.ZU;2-U
Abstract
A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid li brary. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) cl one was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme.