L. Szekely et al., REVERSIBLE NUCLEOLAR TRANSLOCATION OF EPSTEIN-BARR VIRUS-ENCODED EBNA-5 AND HSP70 PROTEINS AFTER EXPOSURE TO HEAT-SHOCK OR CELL-DENSITY CONGESTION, Journal of General Virology, 76, 1995, pp. 2423-2432
The Epstein-Barr virus (EBV)-encoded, nuclear matrix-associated EBNA-5
protein is preferentially localized within distinct nuclear blobs in
EBV-immortalized lymphoblastoid cell lines. We have previously found t
hat the same blobs also contain retinoblastoma (Rb) protein. We now sh
ow that they contain hsp70 protein as well. Both EBNA-5 and hsp70 tran
slocate to the nucleolus under cell density congestion or after heat s
hock. Both proteins relocate to their original position upon the reest
ablishment of normal physiological conditions. EBNA-5 is tightly bound
to the nuclear matrix. The translocated EBNA-5 is also tightly associ
ated with matrix structures, as shown by sequential elution-based cell
fractionation. The Rb protein does not translocate to the nucleolus.
The virally encoded EBNA-1, -2, -3 and -6, and cellular PCNA, snRNP an
d cyclin E are not affected either. The translocation of EBNA-5 to the
nucleolus is not species- or cell type-specific since stress conditio
ns induced the same phenomenon in EBNA-5-transfected human, mouse and
rat cells of different tissue origins.