AN INTEGRATED HIGH-RESOLUTION PHYSICAL MAP OF THE DPC BRCA2 REGION ATCHROMOSOME 13Q12/

We identified a homozygous deletion in a pancreatic carcinoma (DPC) th at localized to a 1-cM region at chromosome 13q12.3, which lay within the 6-cM locus of familial breast cancer susceptibility (BRCA2). Here we present a physical map of the region, consisting of YAC, PAC, and c osmid contigs. The PAC contig comprises 16 clones that together span t he entire BRCA2 region. The PAC contig comprises 22 clones that togeth er span the DPC region. Seventy cosmid clones were localized within an d near the DPC region. Thirty-five sequence-tagged sites were defined and localized within the map. The map indicates the size of the DPC re gion to be near 250 kb, and provides mapped and cloned resources for t he search for the putative tumor suppressor gene(s) in the region.