LIPOPHOSPHOGLYCAN IS PRESENT IN DISTINCTLY DIFFERENT FORM IN DIFFERENT ENTAMOEBA-HISTOLYTICA STRAINS AND ABSENT IN ENTAMOEBA-MOSHKOVSKII AND ENTAMOEBA-INVADENS
G. Srivastava et al., LIPOPHOSPHOGLYCAN IS PRESENT IN DISTINCTLY DIFFERENT FORM IN DIFFERENT ENTAMOEBA-HISTOLYTICA STRAINS AND ABSENT IN ENTAMOEBA-MOSHKOVSKII AND ENTAMOEBA-INVADENS, The Journal of eukaryotic microbiology, 42(5), 1995, pp. 617-622
Lipophosphoglycan has recently been demonstrated on the cell surface o
f Entamoeba histolytica strain HM-1:IMSS. A monoclonal antibody agains
t this molecule had failed to react with some other strains of E. hist
olytica, including the strain Rahman. To determine if a structurally d
istinct lipophosphoglycan existed in Rahman, [H-3]galactose-labeled gl
ycoconjugates were electrophoresed through sodium dodecyl sulfate poly
acrylamide gel electrophoresis. The electrophoretic pattern in Rahman
was very different compared to that obtained with strains HM-1:IMSS an
d 200:NIH. A number of experiments including sensitivity to mild acid,
nitrous acid and phosphoinositol-specific phospholipase C suggest tha
t the Rahman glycoconjugate is indeed a lipophosphogylcan-like molecul
e but distinctly different from that of HM-1:IMSS. Mild acid-treated g
lycoconjugates from Rahman and HM-1:IMSS revealed the presence of neut
ral trisaccharides and monosaccharides in Rahman but not in HM-1:IMSS.
Human immune sera from amoebiasis patients and a polyclonal antibody
against HM-1:IMSS liphophosphoglycan both recognized Rahman glycoconju
gate. Thus, while lipophosphoglycan molecules from the two strains sha
re common epitopes, they are clearly distinct from each other. Molecul
es bearing resemblance to lipophosphoglycan could not be detected in o
ther Entamoeba species, namely Entamoeba invadens and Entamoeba moshko
vskii.