REGENERATION OF PLANTS FROM PRIMARY LEAVES OF COWPEA

Plants were regenerated from the in vitro cultured explants of primary leaves of cowpea (Vigna unguiculata L. Walp). Primary leaves, includi ng the intact petiole, were excised from three-day-old seedlings and c ultured on Gamborg's B5 basal medium containing 8 x 10(-7) M 2,4,5-tri chlorophenoxyacetic acid, 1 x 10(-2) M L-glutamine and 1 x 10(-4) M ad enine sulfate. Callus formed at the petiole end. Prolific shoot regene ration occurred when this callus was transferred to B5 basal medium co ntaining 5 x 10(-6) M 6-benzyl-aminopurine (BAP). Regenerated shoots r ooted in growth-regulator-free B5 basal medium and were established in soil.