INFLUENCE OF MERISTEM-TIP SIZE AND LOCATION ON MORPHOLOGICAL DEVELOPMENT IN DIOSCOREA-CAYENENSIS DIOSCOREA-ROTUNDATA COMPLEX GROSSE-CAILLE AND ONE GENOTYPE OF DIOSCOREA-PRAEHENSILIS
B. Malaurie et al., INFLUENCE OF MERISTEM-TIP SIZE AND LOCATION ON MORPHOLOGICAL DEVELOPMENT IN DIOSCOREA-CAYENENSIS DIOSCOREA-ROTUNDATA COMPLEX GROSSE-CAILLE AND ONE GENOTYPE OF DIOSCOREA-PRAEHENSILIS, Plant cell, tissue and organ culture, 42(2), 1995, pp. 215-218
In vitro morphological development of meristem-tips, excised from micr
oshoots, is reported for one clone 'Grosse Caille' of D. cayenensis-D.
rotundata complex and for one clone of D. praehensilis. The effects o
f size and location of meristem-tip on viability and morphological dev
elopment after 3, 8 and 11 months in vitro culture were studied. The l
arger size of meristem-tip gave the best rooting and plantlet formatio
n while the smaller size gave the best bud production. Axillary buds w
ere more reactive than apical meristem-tips in callusing and rooting w
hen small-sized meristems of the 'Grosse Caille' clone were used; the
use of larger meristem-tips did not induce more differences between ap
ical and axillary buds. The production of plantlets was successful, af
ter 11 months of culture, with a score of 82 and 39% survivors, for 'G
rosse Caille' clone and D. praehensilis genotype, respectively.