R. Bernatzky et al., S-RELATED PROTEIN CAN BE RECOMBINED WITH SELF-COMPATIBILITY IN INTERSPECIFIC DERIVATIVES OF LYCOPERSICON, Biochemical genetics, 33(7-8), 1995, pp. 215-225
Stylar proteins involved in the self-incompatible (SI) response of Lyc
opersicon hirsutum have been identified and mapped to the focus that c
ontrols SI (S locus). L. esculentum, a self-compatible (SC) species of
cultivated tomato, does not display these proteins. Hybrids between S
C L. esculentum and SI L. hirsutum are self-sterile despite these indi
viduals bearing pollen containing the S allele of L. esculentum. In pr
ogeny derived from backcrossing the hybrids to L. esculentum, there wa
s a strong correlation between the presence of the S allele from L. hi
rsutum and self-infertility. However, this relationship was uncoupled
in a number of backcross (BC) progeny. The SI response appeared to be
nonexistent in two self-fertile BC individuals that were heterozygous
for the S allele of L. hirsutum, based on Mendelian segregation of a t
ightly linked DNA marker, CD15, in selfed progeny. Among these progeny
self-fertile individuals that were homozygous for the L. hirsutum all
ele of the linked marker were also determined to be homozygous for an
S-related protein of L. hirsutum through test crosses with L. esculent
um. Therefore, plants were produced that were homozygous for a functio
nal S allele but were self-fertile. This result and other evidence sug
gest that the S-related proteins are not sufficient to elicit a self-i
ncompatible response in L. esculentum and that there is a mutation(s)
in L. esculentum somewhere ether than the S locus that leads to self-c
ompatibility.