HUMAN ERYTHROCYTE CATALASE - 2-D CRYSTAL NUCLEATION AND PRODUCTION OFMULTIPLE CRYSTAL FORMS

Citation
Jr. Harris et A. Holzenburg, HUMAN ERYTHROCYTE CATALASE - 2-D CRYSTAL NUCLEATION AND PRODUCTION OFMULTIPLE CRYSTAL FORMS, Journal of structural biology, 115(1), 1995, pp. 102-112
Citations number
61
Categorie Soggetti
Cell Biology",Biology
ISSN journal
10478477
Volume
115
Issue
1
Year of publication
1995
Pages
102 - 112
Database
ISI
SICI code
1047-8477(1995)115:1<102:HEC-2C>2.0.ZU;2-J
Abstract
Negatively stained electron microscope images are presented, showing t he nucleation of two-dimensional (2-D) crystals of human erythrocyte c atalase produced on mica by the negative staining-carbon film techniqu e. Examples of the formation of partially ordered 2-D arrays and more ordered 2-D crystals are shown and the conditions required for the pro duction of large well-ordered 2-D crystals discussed. The structural t ransformation of one flexuous 2-D paracrystal into a p2 2-D crystal is considered. The crystallographic 2-D image average of this p2 crystal form is presented (lattice parameters a = 9.0 nm, b = 18.6 nm, gamma = 90.8 degrees). It is shown that transmission electron microscopy pro vides the possibility of defining 2-D crystal nucleation, growth of in termediate forms, and low-resolution crystallographic structural analy sis of 2-D crystals of human erythrocyte catalase. Comparison of the v arious electron microscopical negatively stained images with the pepti de backbone of the X-ray structure of bovine liver catalase at differe nt tilt and rotation positions correlates with and emphasizes the mult iple intermolecular contacts and orientations that can be adopted by h uman erythrocyte catalase, leading to various 2-D arrays and 2-D cryst als. Alignment of the surface groups involved in the protein-protein i nteractions that occur during 2-D crystal nucleation and crystal growt h may ultimately be determined. From this approach, when taken togethe r with detailed consideration of protein-solvent and protein-solute el ectrostatic interactions in solution, and at the fluid-air interface, it is considered that a more general theory of crystal nucleation and growth may eventually emerge. (C) 1995 Academic Press, Inc.