Ab. Santoyo et al., PRODUCTION OF L-AMINOACYLASE BY FERMENTATION OF PSEUDOMONAS SP BA2, Journal of chemical technology and biotechnology, 64(2), 1995, pp. 175-180
The growth and enzymatic production of Pseudomonas sp. BA2, a new L-am
inoacylase-producing microorganism, were studied in a bench-top fermen
ter. Multiple fermentations were carried out in order to determine the
optimal pH and temperature values. The influence of the substrate con
centration on both growth and L-aminoacylase activity was also investi
gated. The maximum growth rate and the greatest yield of enzyme were o
btained when the fermentation was carried out at pH 7 . 5, 25 degrees
C and DOT greater than or equal to 50%. N-Acetyl-DL-alanine, at a conc
entration of 20 g dm(-3), was used as the sole carbon and nitrogen sou
rce. The fermentation process provided a maximum biomass concentration
of 3 . 36 g dry weight dm(-3). The highest L-aminoacylase production
(11429 U g(-1) dry weight) was obtained after 39 h of cultivation. The
results were a significant improvement over those previously reported
.