ION-PAIR-SUPERCRITICAL FLUID EXTRACTION OF CLENBUTEROL FROM FOOD SAMPLES

Simultaneous ion-pair/supercritical fluid extraction (SFE) was investi gated as an alternative to liquid solvent extraction for clenbuterol d etermination. Clenbuterol was extracted from diatomaceous earth and fo od matrices (feedstuff, lyophilized milk and liver) as its 10-camphors ulfonate ion-pair using supercritical (SC) CO2 for 30 min of dynamic e xtraction at 383 bar and 40 degrees C. The ion-pair forming reagent wa s added to the extraction cell prior to SFE. Recoveries ranged from 12 to 87% with mean R.S.D. of 15% for triplicate extractions. The use of the ion-pair reagent as a CO2 modifier was also studied in depth.