HYDROGEN-BONDING INTERACTION IN CAPILLARY ELECTROPHORESIS USING POLYETHER MATRICES

Citation
Y. Esaka et al., HYDROGEN-BONDING INTERACTION IN CAPILLARY ELECTROPHORESIS USING POLYETHER MATRICES, Journal of chromatography, 711(2), 1995, pp. 305-311
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
711
Issue
2
Year of publication
1995
Pages
305 - 311
Database
ISI
SICI code
Abstract
Polyethylene glycol (PEG) serves as a novel matrix in capillary electr ophoresis. The purpose of this work was to explore some evidence for h ydrogen-bonding complex formation between analytes and PEG in the sepa ration system using benzoic acids as model analytes. An increase in th e column temperature resulted in a significant decrease in the interac tion between PEG and substituted benzoic acids with hydrogen-donating groups. Addition of urea suppressed the interaction. NMR spectra of ph enol and salicylic acid in the presence of PEG in (CHCl3)-H-2 showed a n obvious electrostatic interaction, probably a hydrogen-bonding inter action, between the hydroxyl protons of the analytes and PEG. These re sults strongly support the contention that hydrogen-bonding interactio n between the polyether segments of PEG and the hydrogen-donating grou ps of analytes occurs in the separation systems. Some other minor inte ractions controlling the separation are also described.