Y. Esaka et al., HYDROGEN-BONDING INTERACTION IN CAPILLARY ELECTROPHORESIS USING POLYETHER MATRICES, Journal of chromatography, 711(2), 1995, pp. 305-311
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Polyethylene glycol (PEG) serves as a novel matrix in capillary electr
ophoresis. The purpose of this work was to explore some evidence for h
ydrogen-bonding complex formation between analytes and PEG in the sepa
ration system using benzoic acids as model analytes. An increase in th
e column temperature resulted in a significant decrease in the interac
tion between PEG and substituted benzoic acids with hydrogen-donating
groups. Addition of urea suppressed the interaction. NMR spectra of ph
enol and salicylic acid in the presence of PEG in (CHCl3)-H-2 showed a
n obvious electrostatic interaction, probably a hydrogen-bonding inter
action, between the hydroxyl protons of the analytes and PEG. These re
sults strongly support the contention that hydrogen-bonding interactio
n between the polyether segments of PEG and the hydrogen-donating grou
ps of analytes occurs in the separation systems. Some other minor inte
ractions controlling the separation are also described.