Tr. Sharma et Jp. Tewari, DETECTION OF GENETIC-VARIATION IN ALTERNARIA-BRASSICAE BY RAPD FINGERPRINTS, Journal of Plant Biochemistry and Biotechnology, 4(2), 1995, pp. 105-107
Genetic Variation in fourteen isolates of Alternaria brassicae collect
ed from different geographical regions of the world was determined by
RAPD (random amplified polymorphic DNA) analysis. Twenty random primer
s were tried to amplify genomic DNA of A. brassicae, Based on the PCR
(polymerase chain reaction) amplification of genomic DNA of A. brassic
ae with four oligonucleotide random primers, fingerprints were generat
ed for each isolate and the amplifed products were compared. Using thi
s technique, intra- and intercontinental genetic variation among isola
tes of A. brassicae could be distinguished.