DETECTION OF GENETIC-VARIATION IN ALTERNARIA-BRASSICAE BY RAPD FINGERPRINTS

Genetic Variation in fourteen isolates of Alternaria brassicae collect ed from different geographical regions of the world was determined by RAPD (random amplified polymorphic DNA) analysis. Twenty random primer s were tried to amplify genomic DNA of A. brassicae, Based on the PCR (polymerase chain reaction) amplification of genomic DNA of A. brassic ae with four oligonucleotide random primers, fingerprints were generat ed for each isolate and the amplifed products were compared. Using thi s technique, intra- and intercontinental genetic variation among isola tes of A. brassicae could be distinguished.